Question: Below is a portion of the template (antisense) strand of DNA that codes for insulin. What is the corresponding mRNA strand transcribed from this DNA? DNA: 3'T A C G C T T A G T A C A T T A A T5' mRNA: What is the amino acid sequence of a protein translated from this mRNA? There are 2 steps to solve this one. The Application of Antisense Technology to Medicine. In a seminal biological insight 50 years ago, James Watson and Francis Crick proposed a structure for the genetic material deoxyribonucleic acid, or DNA. They proposed that DNA consists of two deoxyribonucleotide molecules each having a 5́-end and a 3́-end, which define a polarity for each A. Stoimenow. We give examples of knots with some unusual properties of the crossing number of positive diagrams or strand number of positive braid representations. In particular, we show that Antisense mechanism (A) and phosphorothioate (B).(A) The use of LNA oligonucleotides as inhibitors of disease—forming proteins, or reduction of harmful RNA, is based on specific hybridization to What is the sequence of the antisense strand? Genetics of Stem Cells, Part A Antisense is based on the fact that messenger RNA (mRNA) is in the “sense” direction from 5′ to 3′. Antisense is a limited sequence of DNA in the antisense direction 3′–5′ designed from knowing the sequence of a target gene. The first evidence of an injection of DNA inhibiting RNA activity was shown back in 1977, by impairing the translation of mRNAs in a cell-free system . A year later, the first antisense oligonucleotides used in vivo were created . Zamecnik and Stephenson generated a synthetic 13- nucleotide DNA molecule that targeted a Rous sarcoma virus antisense RNA single-stranded RNA which can hybridize to complementary bases in a target NUCLEIC ACID molecule and inhibit its function. Where the target is the RNA transcript of a gene (i.e. mRNA), copied from the SENSE STRAND of DNA, the antisense RNA is a counter-transcript, copied from the antisense strand (see ANTICODING STRAND of the DNA, and complementary to the mRNA. Antisense technology has been widely used for regulating gene expression. Single-stranded RNA or DNA complementary to a target mRNA can inhibit the translation of the mRNA. Antisense RNA is produced in vivo, while antisense DNA is chemically synthesized as an oligonucleotide, which is extracellularly added to the cells. To maintain the effect of antisense DNA, a synthetic oligonucleotide has Antisense PNAs targeting essential bacterial genes have been shown to possess antibacterial activity via selective inhibition of their targets (reviewed elsewhere (4, 5)). The most commonly studied PNA target is acpP ( 5 , 8 ), which is strongly conserved and one of the most highly expressed genes in UPEC (Figures 1B and 5A ). RNA therapeutics refers to the use of oligonucleotides to target primarily ribonucleic acids (RNA) for therapeutic efforts or in research studies to elucidate functions of genes. Oligonucleotides are distinct from other pharmacological modalities, such as small molecules and antibodies that target mainly proteins, due to their mechanisms of qaS9HQ.